Figure 5.

Validation of the 6OHDA model of Parkinson’s disease. A, Unilateral injection of 6OHDA or vehicle, centered on the SNc. B, Schematic of the cylinder motor assessment. C, Quantification of weight-bearing wall touches measured as a ratio of forelimb use contralateral versus ipsilateral to the injected hemisphere. D, Immunolabeled TH⁺ dopaminergic neurons visualized with DAB in the SNc and VTA. E, Summary of stereological counts of TH⁺ neurons in the SNc and VTA of lesioned or vehicle-injected animals. (vehicle animals, N = 18; 6OHDA animals, N = 17; data are shown as the mean ± SEM. *p < 0.0001. F, Summary stereological counts of TH⁺ boutons in M1 of a subset of animals. Vehicle animals, N = 2; 6OHDA animals, N = 2. Data are shown as the mean ± SEM. *p ≤ 0.05.