| Reagent | Quantity (μl) | Final concentration |
|---|---|---|
| ddH20 | 15 | - |
| Phusion High Fidelity PCR Master Mix with HF buffer (2×) | 25 | 1× |
| Forward primer (10 μM) | 2.5 | 500 nM |
| Reverse primer with T7 promoter sequence (10 μM) | 2.5 | 500 nM |
| Diluted Oligopaint library (−1 ng/μl) | 2.5 | −50 pg/μl |
| EvaGreen dye (20×) | 2.5 | 1× |
| Total | 50 | - |
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