| Reagent | Quantity (μl) |
|---|---|
| T7 reaction | 20 |
| dNTP (10 mM) | 10 |
| RT buffer (5×) | 2 |
| Labeled or unlabeled RT-primer (200 (μM) | 3 |
| Maxima H Minus RT enzyme | 1 |
| RNasin RNase inhibitor | 4 |
| Total | 40 |
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