Figure 1.

Autophagic flux can be measured in primary culture of human leukocytes in vitro. (A) Human blood was fractionated using lymphoprep, and (B) PBMCs and PMNs were cultured without and with CQ in RPMI 1640 (C). Cell populations derived from lymphoprep fractionation were determined using flow cytometry. (D) Western blots for lysosomal system proteins (LC3B and SQSTM1) across PBMCs and PMNs are shown (N = 3, 2 subjects shown in figure). Mr: molecular weight marker