Figure 4.

Co-occurrence of mutations in LC3B and LIR-containing interactors and local effects of mutations on intermolecular interactions. (A) Network of the 70 LIR-containing proteins that interact with LC3B. The plot shows all the LC3B interactors which harbor an experimentally validated (solid line) or predicted LIR with PSSM score > 11 (dashed line). The 39 mutations found in 27 LIR-containing proteins in the proximity or within the LIR motif and co-occurring with LC3B mutations are highlighted in red. Only in one case, we identified an experimentally validated interactions for which no LIR motif could be predicted within the significance threshold used for the prediction, i.e., ATG3, which is showed as a gray edge. (B) Among the 39 co-occurring mutations, 12 were located in the LIR core motif or its proximity (top figure), 6 and 4 were N- and C-terminal to the LIR motif core (middle figure), and 17 resulted in truncations abolishing all or most of the LIR motifs of the interactors (bottom figure)