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P32Q mutation affects LC3B stability and its ability to interact with SQSTM1/p62. (A) Immunoblot of HEK293 cells transfected with GFP, GFP-LC3B WT or GFP-LC3B mutants n = 3 (B) Representative confocal image of U2OS cells transfected with GFP-LC3B and GFP-LC3BP32Q plasmids. n = 3 Scale bar: 5 µm (C) Immunoblot of GFP and SQSTM1/p62 from GFP immunoprecipitation in HEK293 cells transfected with GFP or GFP-LC3B WT or mutants. n = 3
