Figure 3.

circTYW1 inhibits OGD-induced PC12 cell injury. A, expression of circTYW1 in PC12 cells detected by RT-qPCR. PC12 cells after infection were exposed to OGD or PBS for 12 h. B, Caspase-3/7 kit detection of Caspase-3/7 activity in PC12 cells; C, LDH kit detection of cytotoxicity in PC12 cells; D, flow cytometry detection of the PC12 cell apoptosis; E, TUNEL staining of the PC12 cell apoptosis; F, CCK-8 detection of PC12 cell activity. Data were presented as the mean ± SD. All experiments were repeated at least three times, and each experiment was performed in triplicate. One-way (panels A-E) or two-way ANOVA (panel F) with Tukey’s post-hoc test was used for statistical analysis. **p < 0.01 vs PC12 cells treated with normal condition; ##p < 0.01 vs PC12 cells transfected with Lv-NC and exposed to OGD