Figure 7.

CircTYW1 exerts a neuroprotective role during SCI by regulating the ERK1/2 signaling via the miR-380/FGF9 axis. A, western blot detection of ERK1/2 expression and phosphorylation level in rat spinal cord tissues (n = 6); B, staining intensity of phos-ERK1/2 in rat spinal cord tissues by immunohistochemistry (n = 6); C, western blot detection of ERK1/2 expression and phosphorylation levels in PC12 cells. Data were presented as the mean ± SD. All experiments were repeated at least three times, and each experiment was performed in triplicate. One-way (panel B) or two-way ANOVA (panels A and C) with Tukey’s post-hoc test was used for statistical analysis. **p < 0.01 vs sham-operated rats; ##p < 0.01 vs SCI rats injected with Lv-NC; &&p < 0.01 vs PC12 cells treated with normal condition; @@p < 0.01 vs PC12 cells transfected with Lv-NC and exposed to OGD; $$p < 0.01 vs PC12 cells transfected with Lv-circTYW1 + NC and exposed to OGD; %%p < 0.01 vs PC12 cells transfected with Lv-shcircTYW1 + inhibitor NC and exposed to OGD