Figure 8.

Knockdown of FGF9 impairs the protective effect of circTYW1 on PC12 cells. PC12 cells overexpressing circTYW1 were further infected with shRNA targeting FGF9. A, western blot detection of FGF9 protein expression in PC12 cells; B, CCK-8 detection of PC12 cell activity; C, flow cytometry detection of the PC12 cell apoptosis; D, TUNEL staining of the PC12 cell apoptosis. Data were presented as the mean ± SD. All experiments were repeated at least three times, and each experiment was performed in triplicate. Unpaired t test (panels A, C and D) or two-way ANOVA (panel B) with Tukey’s post-hoc test was used for statistical analysis. **p < 0.01 vs Lv-circTYW1 + sh-NC