Figure 8.

Sert degrades both MAPT proteins in vitro via AMPK-induced autophagy. (A) Inducible MAPT cells were treated with DMSO or doxycycline (1 ng/mL) with Sert (1 μM) in a time-dependent manner. Western blot analysis of the levels of p-AMPK and AMPK in cells after each compound treatment. (B) Inducible MAPT cells were pretreated with doxycycline (1 ng/mL) for 24 h. And then, Sert (1 μM) was treated for 48 h. The graph plots the quantification data is shown in (B). n = 3, *p < 0.05, **p < 0.01. (C) Degradation of MAPT oligomers (yellow) was visualized in BiFC cells when cells were co-treated with CC (10 μM) 1 h prior to Sert (1 μM) or Rapa (10 μM). Scale bar: 20 μm. Quantification of data is shown in (C). Values are means ± SEM; n > 20 cells. **p < 0.01, ***p < 0.001. (D) Western blot analysis of the level of p-MAPT (S262), total MAPT, and ACTB in inducible MAPT cells after Sert (1 μM) treatment. The graph plots the quantification data is shown in (D). n = 3, *p < 0.05, **p < 0.01, ***p < 0.001