Figure 8.
Autophagy inhibition alleviated synaptic defects caused by Hsc70-5 knockdown. (A and C) Confocal images of larval NMJ labeled with hrp (green), VGlut, and brp (magenta). Scale bar: 2 μm. (B and D) Quantification of VGlut and brp level at NMJ following Atg1 overexpression and knockdown in elav>Hsc70-5KK100233 background. (E) Confocal images of larval NMJ labeled with brp (magenta) and GluR (green). Arrowheads pointing out regions where presynaptic brp labels were not detected in PSDs. Scale bar: 2 μm (F) Quantification of unapposed glutamate receptor fields. The standard error of mean and standard deviation are shown as a box and a black line. * p < 0.05, *** p < 0.001