Figure 5.

HY5 promotes RGA accumulation under UV-B irradiation. Seedlings were grown in white light for 7 d and exposed to ±UV-B for 4 h

(a–c). mRNA levels of GA metabolism-related genes (GA20ox1, GA3ox1, and GA2ox1) in wild type (WT) and hy5 mutant were quantified by real-time PCR; (d). RGA protein levels in WT, bbx24, rga-24, and hy5-215 after UV-B treatment; and (e). Gray value of RGA protein band (a). Error bars represent the standard error (SE) of the mean of three biological replicates. Different lowercase letters indicate statistically significant differences between the gene expression levels (Tukey’s test; P < 0.05).