Fig. 4.

Specificity of the NESBA for E and N genes of SARS-CoV-2 gRNA. Time-dependent fluorescent signals produced from gene-specific MBs during the NESBA reaction for (a) E and (b) N genes of SARS-CoV-2 or other types of human coronaviruses such as SARS-CoV, MERS-CoV, HCoV-HKU-1, HCoV-229E, HCoV-NL63, and HCoV-OC43. The concentration of SARS-CoV-2 gRNA and other viral RNAs is 5 × 10⁵ copies/μL for all seven human coronaviruses. We obtained the representative lines in (a) and (b) from the triplicate experiments and the reproducibility of the experiments were confirmed by the CV values for T_t in Table S6, which were all smaller than 5%. T_t is the time when the fluorescent signal reaches the threshold (100 a.u.) and CV is calculated as (Standard deviation of T_t)/(Mean of T_t) × 100.