Fig. 6.

Feasibility of the NESBA multiplex detection. Time-dependent fluorescent signals produced from E(I)-MB (green curve) and N(I)-MB-HEX (red curve), which correspond to the respective E and N genes of SARS-CoV-2 gRNA, were simultaneously measured in FAM and HEX channels, respectively ((a): Both E and N genes of SARS-CoV-2 gRNA, (b): E gene of SARS-CoV-2 gRNA, (c): N gene of SARS-CoV-2 gRNA, (d): Blank). The concentration of SARS-CoV-2 gRNA is 5 × 10⁵ copies/μL. We obtained the representative lines in Fig. 6 from the triplicate experiments and the reproducibility of the experiments were confirmed by the CV values for T_t in Table S7, which were all smaller than 5%. T_t is the time when the fluorescent signal reaches the threshold (100 a.u.) and CV is calculated as (Standard deviation of T_t)/(Mean of T_t) × 100. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)