Table 1.
Detection of microthrombi in mice
| Group | Number of animals | Score of microthrombia |
Total | |||
|---|---|---|---|---|---|---|
| 3 | 2 | 1 | 0 | |||
| PBS-treated EPAC1-KO mice | 5 | 0 | 0 | 0 | 5 | 0b |
| PBS-treated WT mice | 5 | 0 | 0 | 0 | 5 | 0 |
| LPS-treated EPAC1-KO mice | 5 | 0 | 0 | 5 | 0 | 5 |
| LPS-treated WT mice | 5 | 0 | 0 | 1 | 4 | 1b |
The left lungs were collected for RNA extraction, and the right lungs were fixed for histopathology studies. Tissue sections of the lungs, livers, and brains were stained with H&E. For animals in which thrombi were detected in the lungs by H&E staining using 100× power microscopy, sections were further validated with immunofluorescence staining against fibrin using the rabbit anti-mouse fibrin(ogen) polyclonal antibody as described (7). Score of 3: microthrombi could be detected in any sections of the above three organs; score of 2: microthrombi could be detected in any sections of the above two organs; score of 1: microthrombi could be detected in only one section of the above organs; score of 0: no microthrombi could be detected in any sections of the above organs. No thrombus was detected in the livers and brains by H&E staining.
p < 0.01, compared with the LPS-treated EPAC1-KO group.