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. 2021 Oct 12;2021:6095963. doi: 10.1155/2021/6095963

Figure 6.

Figure 6

Sa/DDP induces the apoptosis of the HepG2 and SMMC-7721 cells. (a) The HepG2 and SMMC-7721 cells coincubated with Sa (0, 200, 400, and 600 µM) and 5 µM DDP for 72 h were observed for the morphological changes and the Hoechst (blue) staining by using a fluorescence microscope (40x magnification). (b) The percentage of apoptosis of HepG2 and SMMC-7721 cells coincubated with Sa (0, 200, 400, and 600 µM) and 5 µM DDP for 72 h was evaluated by Annexin V-FITC/PI staining and flow cytometric analysis. All results were exhibited as the means ± SD of three different samples. a–fBars with different superscript letters differ significantly (P < 0.05) by Duncan's multiple range test.