Figure 8.

The Sa/DDP treatment induces autophagy of the HepG2 and SMMC-7721 cells. (a) The HepG2 and SMMC-7721 cells coincubated with Sa (0, 200, 400, and 600 µM) and 5 µM DDP for 72 h MDC (green) staining were observed by using a fluorescence microscope (40x magnification). (b) Western blot was used to analyze the expression in the HepG2 and SMMC-7721 cells coincubated with Sa (0, 200, 400, and 600 µM) and 5 µM DDP for 72 h of the autophagy-related proteins p62, Beclin-1, Atg5, and LC3 II, with β-actin as the internal reference. All results were exhibited as the means ± SD of three different samples. ^a–dBars with different superscript letters differ significantly (P < 0.05) by Duncan's multiple range test.