Figure 2.

Calculating total cushion volume and average cushion thickness at the AVJ from volumetric OCT data. A: a 2-D slice from a volumetric OCT image stack. Scale bar = 200 μm. B: manual segmentation of AV superior (green) and inferior (red) cushions. C: 3-D rendering of the two AV cushions. D: the luminal and abluminal surfaces of each cushion were manually labeled. E: 3-D rendering of the luminal surface (green) and abluminal surface (red) of the inferior cushion. F: for every point on the abluminal surface P_{i (1<i<n)}, (n is the number of points on the abluminal surface), the closest point on the luminal surface is found and the distance d_i between the two points is the cushion thickness at point P_i. G: the histogram of cushion thickness calculated at all points. The y-axis is the frequency of a particular cushion thickness value. A, atrium; AVJ, atrioventricular junction; OCT, optical coherence tomography; 2-D, two-dimensional; 3-D, three-dimensional; Vt, ventricle.