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. 2021 Jun 23;321(2):C297–C307. doi: 10.1152/ajpcell.00172.2021

Figure 1.

Figure 1.

Expression profile of Slc12a genes that encode for cation-chloride cotransporters (CCCs). A: gel image showing the RT-PCR results for Slc12a1-7 obtained in a normoxic pulmonary artery (PA) (Pa). PCR amplicons obtained from brain (B) and/or kidney (K) cDNA are given as positive controls. B and C: relative expression levels of Cl−-accumulating cotransporter genes and Cl−-extruding cotransporter genes, respectively, in normoxic (Nx) and chronic hypoxic (CH) PAs determined by quantitative real-time PCR assay (n = 6 rats per group). The error bars show the values of SD. *P < 0.01 vs. normoxic PAs (unpaired t test). †P < 0.05 vs. normoxic Slc12a4/KCC1 and Slc12a6/KCC3 (one-way ANOVA followed by Holm–Sidak test for pairwise comparisons).