Table 1.
RT-PCR primer sequences
| Gene | Accession No. | Primers | Sequence (5′–3′) | Predicted Size, bp |
|---|---|---|---|---|
| Slc12a1 | NM_001270617.1 | Forward | AGGCACGATCGATGTTTGGT | 457 |
| Reverse | CGCCACTGGAAGGCTCAATA | |||
| Slc12a2 | NM_031798.1 | Forward | CTGGTATTCTAGCTGGGGCG | 781 |
| Reverse | CCCCAGTTCACATCTGGCTT | |||
| Slc12a3 | NM_019345.3 | Forward | CAGCACCTTGTACCTACGCA | 496 |
| Reverse | AGGTCCCAGACTCCGAGAAA | |||
| Slc12a4 | NM_019229.2 | Forward | AAGACTCGGATGGACAGGGT | 486 |
| Reverse | ATTCTGGTCCCAAAGAGCGG | |||
| Slc12a5 | NM_134363.1 | Forward | GGACCCCCGCATACAAAGAA | 570 |
| Reverse | CCTCCAGACCTTGTGGTGAC | |||
| Slc12a6 | NM_001109630.1 | Forward | TCCAGAAACCACCACCAAGAT | 689 |
| Reverse | ACATTGTACAGCAGCAGCAGAT | |||
| Slc12a7 | NM_001013144.2 | Forward | AGCCATGCCCACGAACTTTA | 509 |
| Reverse | TGGCTGTCAGCATTGTACAGG | |||
| Slc4a1 | NM_012651.2 | Forward | GGCCCCTTCTGAACCTCTTC | 339 |
| Reverse | ACGCGGAACACTCTTTCTGT | |||
| Slc4a2 | NM_017048.2 | Forward | CCCAGGGTCCCTACTGATGA | 700 |
| Reverse | GCTCACGCTCTCTATCCACC | |||
| Slc4a3 | NM_017049.1 | Forward | AGAAGATCCCTGAGGACGCT | 679 |
| Reverse | TTCTCTCCTAGCAGACCCCC | |||
| Slc4a8 | NM_199497.2 | Forward | GGGACCAGCACGATTCTCAA | 323 |
| Reverse | TTCCATTCGGCTTCTTCCCC |