Table 2.
Real-time PCR primer sequences
| Gene | Accession No. | Primers | Sequence (5′–3′) | Predicted Size, bp |
|---|---|---|---|---|
| Slc12a1 | NM_001270617.1 | Forward | AATGCAGTGGCTGTCGCTAT | 131 |
| Reverse | ATCACCACCGTGATGGAACC | |||
| Slc12a2 | NM_031798.1 | Forward | GGAGCGATTCTCTGCTGCAT | 122 |
| Reverse | CCCCAGTTCACATCTGGCTT | |||
| Slc12a3 | NM_019345.3 | Forward | CAGCACCTTGTACCTACGCA | 138 |
| Reverse | TTGAGGAACGAGTGCAGGTC | |||
| Slc12a4 | NM_019229.2 | Forward | TCGGATGGACAGGGTAACCA | 150 |
| Reverse | AAGCTTGCCCAGGAGAGATG | |||
| Slc12a5 | NM_134363.1 | Forward | AAGGACCCCCGCATACAAAG | 146 |
| Reverse | GACAGAGCCCACAATGGTCA | |||
| Slc12a6 | NM_001109630.1 | Forward | TGCCAGAAACAAGCCGTAGT | 134 |
| Reverse | GTTCTGATTCGGGTCCTCGG | |||
| Slc12a7 | NM_001013144.2 | Forward | ATGCCCACGAACTTTACGGT | 135 |
| Reverse | GTTTCCATCTCCCGGCGTAG | |||
| Slc4a1 | NM_012651.2 | Forward | GCGCCGGTATCCTTACTACC | 130 |
| Reverse | CTTTTCTCCCAGGAGACCGC | |||
| Slc4a2 | NM_017048.2 | Forward | AAACACAGCCACCCAAGTGA | 137 |
| Reverse | CCGATGAGAGGCTCAGTGAC | |||
| Slc4a3 | NM_017049.1 | Forward | TTGGGGAATCACCACCCAAC | 122 |
| Reverse | AGGGGCTTCTCCTTTGCATC | |||
| Slc4a8 | NM_199497.2 | Forward | AAGCCAACGAGGGATGATCG | 148 |
| Reverse | TCCGGTTAATGATGACGGCG |