Table 1.

Computer-assisted measurements of motility and morphology parameters of dividing cells

Interval 1 (from the start of anaphase to the start of cytokinesis)
Cell Line	Numbers of Cells	Total Elapsed Time, min	Mean Instantaneous Velocity, μm/min	Mean Maximal Length, μm	Mean Area, μm2	Mean Perimeter, μm	Mean Radial Length, μm	Mean Convexity, °	Mean Concavity, °
WT SM3a	10	9.4 ± 1.43	0.14 ± 0.01	10.91 ± 0.38	60.47 ± 2.35	29.99 ± 0.74	4.47 ± 0.09	691 ± 26	331 ± 26
AS#I-5b	10	9.95 ± 1.15	0.16 ± 0.01	11.9 ± 1.32	65.86 ± 7.67	31.72 ± 2.59	4.7 ± 0.32	725 ± 41	365 ± 41
S#105c	10	8.45 ± 2.02	0.23 ± 0.04	16.05 ± 2.16	82.0 ± 8.1	40.01 ± 4.26	5.63 ± 0.46	813 ± 67	453 ± 67
P value	a vs. b	0.77	0.21	0.47	0.62	0.79	0.85	0.49	0.49
	a vs. c	0.34	0.02	0.02	0.02	0.02	0.02	0.24	0.24
	b vs. c	0.14	0.10	0.08	0.17	0.09	0.11	0.28	0.28

Interval 2 (from the start of cytokinesis to 50% cytokinesis)
Cell Line	Numbers of Cells Analyzed	Total Elapsed Time, min	Mean Instantaneous Velocity, μm/min	Mean Maximal Length, μm	Mean Area, μm2	Mean Perimeter, μm	Mean Radial Length, μm	Mean Convexity, °	Mean Concavity, °
WT SM3a	10	8.1 ± 0.68	0.21 ± 0.02	12.49 ± 0.49	63.48 ± 2.7	33.77 ± 1.07	4.65 ± 0.11	867 ± 49	510 ± 50
AS#I-5b	10	6.6 ± 0.56	0.27 ± 0.04	14.15 ± 1.01	74.27 ± 7.15	37.97 ± 2.25	5.05 ± 0.27	1013 ± 56	650 ± 55
S#105c	10	6.4 ± 0.73	0.25 ± 0.03	16.85 ± 2.18	86.6 ± 9.06	43.76 ± 4.11	5.75 ± 0.47	1046 ± 74	686 ± 74
P value	a vs. b	0.11	0.39	0.16	0.43	0.11	0.19	0.07	0.08
	a vs. c	0.10	0.23	0.12	0.09	0.08	0.09	0.06	0.06
	b vs. c	0.79	0.65	0.57	0.30	0.23	0.21	0.72	0.70

WT SM3, antisense calponin 2 cDNA transfected (AS#I-5) and sense calponin 2 cDNA transfected (S#105) cells were studied using 2D-DIAS. The values are presented as means ± SD and statistical analysis was performed using Student’s t test after testing normality and equal variance. Mann–Whitney rank sum test was used where normality or equal variance failed in Student’s t test. P < 0.05 is considered significant (indicated with bold font).