Figure 1.
FACS analysis of GFP expression in 293T Lenti-X cells transduced with SIV-based lentiviral vectors
Cells were transduced with equal amounts (3 × 105 RT counts) of integrating LVs (top) or IDLVs (bottom) delivering GFP. GAE, parental self-inactivating (SIN) SIV-based transfer vector; GAEdPPT, mutant transfer vector with 3′ PPT deleted; GAEdPPTdU3, double mutant with an extended 423-bp U3 deletion and with 3′ PPT deleted; GAEdU3, mutant transfer vector with an extended 423-bp U3 deletion; GFP, green fluorescent protein; MFI, mean fluorescence intensity.