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. 2021 Oct 1;23:263–275. doi: 10.1016/j.omtm.2021.09.011

Figure 1.

Figure 1

FACS analysis of GFP expression in 293T Lenti-X cells transduced with SIV-based lentiviral vectors

Cells were transduced with equal amounts (3 × 105 RT counts) of integrating LVs (top) or IDLVs (bottom) delivering GFP. GAE, parental self-inactivating (SIN) SIV-based transfer vector; GAEdPPT, mutant transfer vector with 3′ PPT deleted; GAEdPPTdU3, double mutant with an extended 423-bp U3 deletion and with 3′ PPT deleted; GAEdU3, mutant transfer vector with an extended 423-bp U3 deletion; GFP, green fluorescent protein; MFI, mean fluorescence intensity.