Figure 3.

DNA recombination frequencies of SIV-based IN-competent and IN-defective lentiviral vectors

(A) Average geneticin-resistant colony formation titer assay results in transduced HeLa cells comparing the frequency of integration among integrase-competent (LV, black bars) and integrase-defective (IDLV, gray bars) lentiviral vector. (B) Ratio between the number of colonies of LV- and IDLV-transduced HeLa cells from (A). GAE, parental lentiviral vectors; GAEdPPT, vectors with a 3′-PPT deletion; GAEdPPTdU3, vectors with a 3′-PPT deletion and a large U3 deletion; GAEdU3, vectors with a large U3 deletion. y axis in (A) represents number of colonies/10⁶ RT counts of vector supernatant, while in (B), it represents the LV/IDLV ratio among colonies. The error bars indicate the standard deviation among n = 4 independent experiments, and p values are shown for intergroup comparisons. ∗∗p < 0.01, ∗∗∗p < 0.001.