Figure 5.

DNA recombination frequencies of SIV-based episomal transfer vectors

Average geneticin-resistant colony formation titer assay results in transduced HeLa cells comparing the frequency of integration among the episomal lentiviral transfer vectors. y axis represents infectious units/10⁶ RT counts of vector supernatant. The error bars indicate the standard error among n = 4 independent experiments, and p values are shown for intergroup comparisons. ∗∗p < 0.01, ∗∗∗p < 0.001. eGAE, parental episomal lentiviral vector; eGAEdPPT, episomal vector with a 3′ PPT deletion; eGAEdPPTdU3, episomal vector with a 3′-PPT deletion and a large U3 deletion; eGAEdU3, episomal vector with a large U3 deletion; eSH, episomal vector with full-length LTR.