Figure 1.

MELTF-AS1 is upregulated in osteosarcoma and is associated with distant metastasis of osteosarcoma

(A) Heatmap of lncRNAs differentially expressed in osteosarcoma with or without distant metastasis. Red indicates upregulation and green indicates downregulation. The red underline denotes MELTF-AS1. (B) The comparison of MELTF-AS1 expression between osteosarcoma tissues with or without distant metastasis in the TARGET database. (C) MELTF-AS1 expression in 40 pairs of osteosarcoma and corresponding normal tissues detected by qRT-PCR. (D) Kaplan-Meier survival analysis of osteosarcoma patients’ overall survival based on MELTF-AS1 expression. The median level of MELTF-AS1 is used as the cutoff (n = 40, p = 0.0195). (E) MELTF-AS1 expression in osteosarcoma cell lines (SaoS-2, 143B, MG63, U2-OS, HOS, and NIH 3T3), Ewing’s sarcoma cell lines (A-673 and Hs863.T), and osteoblast cells hFoB1.19 detected by qRT-PCR. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.