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. 2021 Aug 26;26:787–797. doi: 10.1016/j.omtn.2021.08.022

Figure 2.

Figure 2

RREB1 binds to the promoter region of MELTF-AS1 and activates its transcription in MELTF-AS1

(A and B) qRT-PCR analysis of MELTF-AS1 expression in 143B and MG63 cells following RREB1 knockdown and overexpression. (C) qRT-PCR analysis of RREB1 expression in 40 pairs osteosarcoma tissues and corresponding adjacent normal tissues. (D) In situ hybridization (ISH) analysis of RREB1 expression in 40 pairs osteosarcoma tissues and corresponding adjacent normal tissues. (E) ChIP-qRT-PCR analysis of RREB1 occupancy in the MELTF-AS1 promoter in 143B and MG63 cells. (F) Luciferase reporter assays of the 143B cells transfected with reporter vector containing full length of the MELTF-AS1 promoter or containing the promoter with deletion of binding sites, the pcDNA-RREB1 vector, or an empty vector. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.