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. 2021 Aug 26;26:787–797. doi: 10.1016/j.omtn.2021.08.022

Figure 5.

Figure 5

MELTF-AS1 promotes MMP14 expression through adsorbing miR-485-5p

(A) Dual luciferase reporter assays were conducted in the 143B cells co-transfected with the miR-485-5p mimics and luciferase reporter plasmids that were inserted with the wild-type MMP14 3′ UTR or binding sites-mutated MMP14 3′ UTR. (B) qRT-PCR analysis of MMP14 expression after knockdown of MELTF-AS1 or knockdown of MELTF-AS1 combining inhibition of miR-485-5p in 143B cells. (C) qRT-PCR analysis of MMP14 expression after overexpressing MELTF-AS1 or overexpressing MELTF-AS1 combining overexpressing miR-485-5p in 143B cells. (D) MMP14 expression was detected by western blot in 143B cells with indicated treatments. (E) qRT-PCR analysis of MMP14 expression in 40 pairs osteosarcoma tissues and corresponding adjacent normal tissues. (F) Correlation analysis between MELTF-AS1 and miR-485-5p (r = 0.-447, p = 0.004), MMP14 and miR-485-5p (r = −0.378, p = 0.016), and MELTF-AS1 and MMP14 (r = 0.440, p = 0.005) in 40 paired osteosarcoma samples. ∗∗p < 0.01; ∗∗∗p < 0.001.