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. 2021 Jun 28;386(1):29–45. doi: 10.1007/s00441-021-03482-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Workflow for reconstruction of neuropils from micro-CT data of Bombus terrestris. (a) Virtual frontal 2D slice from the micro-CT data showing the position of the brain in the head capsule. Red arrows: muscles; green arrows: retina. (b) Same slice as in (a) but cropped to exclude all non-neuronal tissues. (c) The cropped slice with all neuropil regions of interest manually labeled. The color code of the neuropils is consistent with Brandt et al. (2005) and Kurylas et al. (2008). (d) Surface model of an individual brain reconstruction with all neuropils labeled. The remaining neuropils (RN) were labeled semi-transparent to make the central complex (CX) better visible. Neuropils: antennal lobes (AL), anterior optic tubercle (AOTU), basal ring (BR) central complex (CX), collar (CO), lamina (LA), lip (LIP), lobula (LO), medulla (ME), ocellar synaptic plexi (OC), peduncle (PED), and remaining neuropils (RN). Scale bars = 1000 µm