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. 2021 Sep 25;23:181–191. doi: 10.1016/j.omto.2021.09.004

Figure 1.

Figure 1

Evaluation of the ASCL1 knockdown and its effects on CRC factors by Sh6 and Sh8 in Kelly

Sh6 and Sh8 induced knockdown of ASCL1 in the presence of doxycycline (Dox) at day 4, as assessed by (A and B) RT-PCR and (C and D) western blot analysis. (E and F) Gene expression of ASCL1, SOX10, and GATA3 in Sh6- and Sh8-inducible Kelly cells by RT-PCR analysis. (G and H) Protein expression of CRC transcription factors in Sh6 and Sh8 cells. (All statistical data presented as mean ± SEM with n = 3, ∗∗p < 0.01 or ∗p < 0.05.)