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Effect of ASCL1 knockdown and treatment of ATRA on proliferation and differentiation
(A and B) The gene expression of ASCL1 and TH in day-3 treatments of Dox versus Dox + ATRA in Sh6- and Sh8-inducible cells by RT-PCR assay. (C) Protein expression of ASCL1 and TH as assessed by western blots. (D) Proliferation percentage in Sh6- and Sh8-inducible cells at day-4 treatment of Dox and Dox + ATRA combination as measured by CyQuant (n = 3, ∗∗p < 0.01 or ∗p < 0.05).
