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Effect of JQ1 and ATRA combinatorial treatments on tumor-sphere formation
(A) Tumor-sphere formation in 25 days after treating cells with JQ1, ATRA, and combinations from 10 nM to 1 μM. (B) Proliferation inhibition measured by CyQuant assay of Kelly with JQ1 and ATRA combinatorial treatments for 10 days. Representative images of tumor spheres and proliferating colony of Kelly cells. Cells were imaged after adding crystal violet dye and the washing procedure.
