Figure 6.

CD47-blockage in polyplex-transfected tumors

13-day-old, palpable 231/LM2-4-EGFPluc tumors were transfected with LPEI-based nanoparticles loaded with psCV1-hIgG1 or pmCherry at N/P ratio 9 by injection into the middle of the tumor core (from two puncture sides per tumor). Tumors were harvested 24 h after injection and stained for huCD47. (A) Gradient CD47 staining increasing from the injection site to the periphery of a psCV1-hIgG1-transfected tumor. (B) CD47 staining of a pmCherry-transfected tumor. Asterisk, necrotic area. Image stitching with zoomed-in images at 40×. Scale bars: 2 mm (stitched overview pictures), 20 μm (zoomed-in pictures).