Figure 7.

Intratumoral psCV1-hIgG1 transfection triggers macrophage infiltration

Macrophage staining of in vivo transfected 231/LM2-4-EGFPLuc tumors. (A) IHC staining with an anti-F4/80 antibody murine macrophage marker of tumor tissue harvested 12 h, 17 h, or 24 h after in vivo transfection by intratumoral injection of polyplexes formulated with psCV1-hIgG1- or pmCherry-expressing pDNA into 13-day-old tumors; 40× magnification, scale bar: 25 μm. (B) Semiquantitative analysis of macrophage staining signal shown in bar charts (in %); n = 4–6 + SD. ∗p < 0.05; U test (Mann-Whitney).