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PKR is required for the enhanced activation of NF-κB by TNF-α and IFN-γ. Pkr+/+ and Pkr0/0 cell lines were treated with TNF-α (T) (0.025 ng/ml) or IFN-γ (I) (1,000 U/ml) or cotreated with both (T/I) for 1 h or not treated (−) (control). Whole-cell extracts were subjected to EMSA.
