FIG. 7.

PKR interacts with IKK complex in vivo. Cells (293T) were transiently transfected with 2 μg of plasmids expressing a catalytically inactive mutant of human PKR or human Stat6 as described in Materials and Methods. (A) IKK complex was immunoprecipitated from extracts (0.5 mg [lane 6] or 1 mg [lanes 2 to 4 and 7 to 8]) using an anti-IKKα antibody, and the immune complex was subjected to Western blotting for PKR or Stat6 as indicated. A rabbit IgG polyclonal antibody was used as the control. The blots were subsequently stripped and reprobed for IKKα. (B) PKR and Stat6 were immunoprecipitated from PKR- or Stat6-overexpressing extracts (1 mg), respectively, and the immunocomplexes were subjected to analysis by Western blotting for endogenous IKKα.