FIG. 8.

Dominant negative IKKβ inhibits pIC-stimulated NF-κB-dependent transcription in a dose-dependent manner. T98G cells were transiently cotransfected with an NF-κB-dependent luciferase reporter (5 NF-κB sites) and increasing amounts of a mutant IKKβ expression plasmid or the corresponding empty vector as the control. Approximately 36 h posttransfection, cells were treated with pIC (100 μg/ml for 8 h) and extracts were prepared and analyzed for luciferase activity. The graph reflects firefly luciferase units corrected for transfection efficiency by expression from a Renilla luciferase plasmid included as an internal control in the dual-luciferase assay system. The experiment was performed in duplicate and is representative of several separate experiments.