Figure 2.

EGF-JNK signaling regulates BIRC6 stability

(A) After serum starvation for 48 h, cells were treated with EGF for 30 min, followed by immunoblotting as indicated. (B) After serum starvation, Hs578T cells were treated with or without SP600125 (10 μM) for 1.5 h and then treated with EGF for 30 min. Equal amounts of cell lysates were immunoblotted with the indicated antibodies. (C) Hs578T cell lysates were immunoprecipitated with an anti-BIRC6 antibody, followed by immunoblotting as indicated. (D) Hs578T cells were treated with or without EGF (100 ng/mL) and then treated with MG132 (10 μM) for another 6 h. Cell lysates were immunoprecipitated with an anti-BIRC6 antibody, followed by immunoblotting as indicated. (E) Hs578T cells were treated with or without SP600125 (10 μM) and then treated with cycloheximide (CHX, 100 μg/mL) for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (F) After serum starvation, Hs578T cells were treated with or without EGF and then treated with CHX (100 μg/mL) for the indicated times. Cell lysates were immunoblotted as indicated.