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. 2021 Sep 28;26:798–812. doi: 10.1016/j.omtn.2021.09.011

Figure 4.

Figure 4

BIRC6 promotes TNBC cell growth and tumorigenesis by blocking SMAC-mediated apoptosis

(A–D) Immunoblotting analysis (A), MTT assay showing growth (B), colony formation assay (C), and tumorsphere assay (D) were performed in Hs578T cells transduced with scramble or BIRC6 shRNA. (E) The growth curves of xenografts derived from MDA-MB-468 cells transduced with scramble or BIRC6 shRNA. (F–I) Immunoblotting analysis (F), MTT assay showing growth (G), colony formation assay (H), and tumorsphere assay (I) were performed in Hs578T cells with BIRC6 KO or BIRC6 re-expression. (J) Representative plots of cell apoptosis analysis in Hs578T cells with or without BIRC6 knockdown. (K–M) MTT assay showing growth (K), colony formation assay (L), and immunoblotting analysis (M) were performed in Hs578T cells transduced with the indicated shRNAs. (N) The growth curves of xenografts derived from Hs578T cells transduced with the indicated shRNAs. (O) Representative IHC staining for BIRC6, SMAC, and cleaved caspase-3 in tumor tissues from mice. Data are means ± SD. ∗∗p < 0.01, ∗∗∗p < 0.001.