Figure 6.

pCLNs can efficiently deliver BIRC6-siRNA into TNBC cells in vitro and in vivo

(A) Schematic diagram of the synthesis process for pCLN/siRNA. (B) Size distributions and transmission electron microscope (TEM) images of pCLNs (left) and pCLN/siRNA (right). Scale bar in TEM images: 100 nm. (C) Intracellular fluorescence intensities of Cy3-siRNA were determined by flow cytometry after MDA-MB-468 cells were incubated with free Cy3-siRNA or pCLN/Cy3-siRNA for the indicated times. (D) Intracellular localization and lysosomal escape were observed by confocal laser scanning microscopy (CLSM) after MDA-MB-468 cells were incubated with pCLN/Cy3-siRNA for the indicated times. (E) In vivo imaging of MDA-MB-468 xenograft-bearing mice after tail vein injection of free Cy3-siRNA or pCLN/Cy3-siRNA for the indicated times.