Figure 4.

Exosomal miR-320b promotes lymphatic metastasis in vivo

(A) Schematic representation of the popliteal LN metastasis nude mice model establishment. (B) Representative bioluminescent images of popliteal metastatic LN from nude mice treated with PBS, KYSE150-EXO_Vector, or KYSE150-EXO_miR-320b (n = 5). Red arrow indicates footpad tumor and metastatic popliteal LN. (C) Representative image of the popliteal LN metastasis model. Representative images of enucleated popliteal LNs (D) and histogram analysis (E) of the LN volume of all groups (n = 5). (F) Representative images of IHC staining with anti-luciferase antibody (n = 5). Scale bars, 50 μm. (G) Percentages of LN metastasis in all groups (n = 10). (H) Staining of LYVE-1 protein in xenograft model primary tumor tissues. The lymphatic vessels are indicated by red arrows. Scale bar, 50 μm. (I) Kaplan-Meier survival curve for PBS, KYSE150-EXO_Vector, or KYSE150-EXO_miR-320b groups (n = 10). ∗∗∗p < 0.001.