Figure 6.

miR-320b overexpression is correlated with ESCC LN metastasis through promoting ESCC malignant phenotypes

(A) TCGA database analysis of miR-320b expression in EC and adjacent normal tissues. (B) Survival curves of EC patients with alteration miR-320b levels was calculated in Kaplan-Meier method. (C) qRT-PCR analysis to exanimate the miR-320b expression in KYSE30, KYSE150, TE-1, EC109, EC9706, and HET-1A cells. (D) Cell proliferation was examined by CCK-8 assay in KYSE150 cell with indicated treatment. (E) Colony formation assay of KYSE150 cells followed by indicated treatment. (F) EdU proliferation assay of KYSE150 cells followed by indicated treatment. Scale bars, 50 μm. (G) Migration and invasion ability of indicated treatment cells were assessed by Transwell assay. (H) Motility ability of indicated treatment cells were assessed by wound healing assays. Scale bars, 100 μm. (I) Western blot analysis of protein E-cadherin (E-cad) and Vimentin (Vim) expression in indicated treatment cells. ∗∗∗p < 0.001.