Table 3.
Diversity estimation of the 16S rDNA gene libraries of the cecum and duodenum in laying hens reared on the LRS and NRS.
| Group | Effective reads | Average length | OTUs | Simpson | Shannon | ACE | Chao | Good's coverage |
|---|---|---|---|---|---|---|---|---|
| LC, n = 10 | 81,873.89 ± 8,718.91 | 433.64 ± 30.73 | 1,244.67 ± 43.52a | 0.99 ± 0.00 | 7.67 ± 0.18a | 1,421.53 ± 62.25a | 1,386.97 ± 63.01a | 0.99 ± 0.00 |
| NC, n = 10 | 78,116.33 ± 9,800.69 | 429.55 ± 35.75 | 1,188.33 ± 59.09a | 0.98 ± 0.01 | 7.51 ± 0.24a | 1,357.05 ± 68.20a | 1,328.05 ± 62.56a | 0.99 ± 0.00 |
| LD, n = 10 | 90,437.11 ± 5,143.49 | 441.22 ± 41.46 | 854.67 ± 77.90b | 0.83 ± 0.16 | 5.12 ± 0.70b | 991.64 ± 84.41 | 986.73 ± 60.46b | 0.99 ± 0.00 |
| ND, n = 10 | 87,740.44 ± 9,487.34 | 437.92 ± 45.51 | 815.56 ± 66.37b | 0.86 ± 0.15 | 4.90 ± 0.63b | 941.91 ± 70.79 | 939.11 ± 74.72b | 0.99 ± 0.00 |
Operational taxonomic units (OTUs) were defined at 3% dissimilarity. Richness estimators (ACE and Chao) and diversity indices (Shannon) were calculated. LRS, floor litter housing system; NRS, plastic net housing system; LC, cecum of laying hens reared in the LRS; NC, cecum of laying hens reared in the NRS; LD, duodenum of laying hens reared in the LRS; ND, duodenum of laying hens reared in the NRS.
a,b
Means with different superscripts within each column are significantly different (p < 0.05).