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. 2021 Oct 19;12(11):964. doi: 10.1038/s41419-021-04250-1

Fig. 4. miR-4523 overexpression ameliorates DEX-induced apoptosis in human osteoblasts.

Fig. 4

Primary human osteoblasts (A–F) or hFOB1.19 osteoblastic cells (G and H), stably expressing a lentiviral construct encoding the pre-miR-4523 (“lv-pre-miR-4523”) or control miR construct (lv-miRC), were established. Cells were then treated with dexamethasone (DEX, 1 μM) or vehicle control (“Veh”), and cultured for applied time periods. Cell viability was tested by MTT assays (A and H). Caspase-3 and caspase-9 activities (B and C) as well as expression of apoptosis-associated proteins (D) and histone-bound DNA contents (E) were tested. Cell apoptosis was examined by nuclear TUNEL staining (F and I) and Annexin V FACS (G) assays, with results quantified. Data were presented as mean ± standard deviation (SD, n = 5). *P < 0.05 versus “Veh” treatment in “lv-miRC” cells; # P < 0.05 versus “DEX” treatment in “lv-miRC” cells. The experiments were repeated five times with similar results obtained.