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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: J Invest Dermatol. 2021 Apr 20;141(11):2577–2586. doi: 10.1016/j.jid.2021.03.025

Figure 5: NPS-R568 normalizes e-cadherin and calcium response after scratch wounding).

Figure 5:

(a) NHEK and AHEKs before and 100 seconds after scratch. Red hue denotes higher [Ca2+]i. Scale-bar = 200 μm. (b) Time-traces of percentage area with increased [Ca2+]i after scratch . Representative of 5–8 experiments on cells from 3 neonatal and 3 aged donors per group. (c) Distribution of maximum calcium response area after scratch. NHEK (blue) and AHEK (red) treated with vehicle, 0.5 μM NPSR-568 (yellow), or 1 μM NPS R-568 (green). (d) Distribution of single cell [Ca2+]i response to increased extracellular calcium. Asterisks indicate statistical significance with p<0.05. N=192–409 cells per group from 6–10 experiments on cells from 3 neonatal and 3 aged donors. (e) Immunofluorescence E-cadherin staining (red) of NHEK and AHEK monolayers switched to 1.2mM [Ca2+] media containing 0 (DMSO vehicle), 0.5, or 1μM NPS-R568 for 15 minutes. Images are representative of 3 separate experiments on cells from 3 neonatal and 3 aged donors. Scale-bar = 20 μm. (f) Quantification of e-cadherin levels at the plasma membrane. Asterisks denote statistically significant difference from NHEKs levels determined via a two tailed t-test with p<0.05.