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. 2021 Oct 19;12:6000. doi: 10.1038/s41467-021-26209-8

Fig. 3. Select high-impact gut metabolites and pathways as regulated by microbiota.

Fig. 3

a Variable importance plot of top 50 fecal metabolites (y-axis) ranked by contribution to mean decrease accuracy of Gini coefficient (x-axis) in the random forest model for discerning group difference. b Diagram summary of perturbed fecal pathways of phenylalanine, tyrosine, and tryptophan biosynthesis and metabolism, with significantly altered metabolites labeled in red (GF>CONV-R) or blue (GF<CONV-R) (two-sided Welch’s t-test, fold change ≥ 1.5, p < 0.01). c, d Box and Whisker plots of fecal indoles (c) and bile acid profiles (d) as synthesized or mediated by microbiota, with the box ranging from the first quartile to the third while the whiskers going from each quartile to the minimum or maximum (GF, N = 12; CONV-R, N = 12), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-sided Welch’s t-test; exact p values and adjusted p values (i.e., q values) are provided in Supplementary Data 2. SM sphingomyelin, Phe L-phenylalanine, Tyr L-tyrosine, Trp L-tryptophan, 5-HT 5-hydroxytryptamine, NAS N-acetylserotonin, 5-MIAA 5-methoxyindole-3-acetate, Kyna kynurenate, Kyn L-kynurenine, AA anthranilate, XA xanthurenate, IAA indole-3-acetate, IPA indole-3-propionate, ILA indole-3-lactate, I3A indole-3-carboxaldehyde, L-DOPA L-3,4-dihydroxyphenylalanine, DHICA 5,6-dihydroxyindole-2-carboxylate, CDCA chenodeoxycholate, CA cholate, αMCA α-muricholate, TCDCA taurochenodeoxycholate, TCA taurocholate, TαMCA tauro α-muricholate, DCA deoxycholate, HDCA hyodeoxycholate, LCA lithocholate.