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Northern blot analysis of MDM2 mRNA after X-ray treatment. X-ray (12 Gy) treatment induces only very low levels of p21WAF1/CIP1 mRNA in Rat-1 cells at either 2 or 24 h postirradiation. This induced level reflects the background level of expression observed in REF52 cells, which show highly elevated levels of p21WAF1/CIP1 mRNA after X irradiation.
