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p21WAF1/CIP1 expression and a G1 arrest are restored after X-ray treatment of P13.5 cells, a clone of Rat-1 cells stably transfected with a 120-kb PAC containing a genomic copy of the rat p21WAF1/CIP1 gene. (A) A low level of background p21WAF1/CIP1 expression was observed in P13.5 cells which is significantly increased after X irradiation (12 Gy). Sizes are indicated in kilodaltons. (B) P13.5 cells exhibit a G1 arrest 7 h after X irradiation (12 Gy).
