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. 2021 Oct 19;12:6093. doi: 10.1038/s41467-021-26337-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Six CI2 variants were co-expressed with the protein folding sensor (pSEVA631(Sp)-lbpAp-GFP-ASV), and the GFP fluorescence was analyzed by FACS. The average mean GFP fluorescence was compared to the Gibbs free energy of unfolding (∆G_unf) determined from global fits of thermal and chemical unfolding of each protein. All measurements were determined in triplicates and the data are presented as mean values ± standard deviation. Source data are provided as a Source Data file.