Skip to main content
. 2021 Oct 19;12:6093. doi: 10.1038/s41467-021-26337-1

Fig. 6. PARP1-BRCT mutants with changed folding properties identified from a randomly generated mutant library using the dual-reporter system.

Fig. 6

a Correlation between translation levels of 20 PARP1-BRCT mutants quantified from Western blots (gray, n = 1) and flow cytometry analysis of mean mCherry fluorescence values ± standard deviation (red), each normalized to the WT signal (n ≥ 3, biologically independent samples). b GFP levels analyzed using flow cytometry as a measure for protein solubility and folding properties. Data are presented as mean values ± standard deviation for n ≥ 3 biologically independent samples. c Percentage of soluble protein determined by Western blot for the 9 PARP1-BRCT mutants with a detectable GFP response signal. Western blot analysis of total protein yield (tot) and soluble protein (sol) after fractionated cell disruption (n = 1). Source data are provided as a Source Data file.